Kvasny Prum. 1963; 9(6): 147-149 | DOI: 10.18832/kp1963026

Quantitative determination of saccharose and raffinose and of some reducing sugars.Peer-reviewed article

M. TROJNA, J. HUBÁČEK

The article deals with the quatitative chromatographic analysis of reducing and non-reducing sugars [saccharose, raffinose]. Divided non-reducing oligosaccharides are exposed on paper to enzymatic hydrolysis by β-fructosidase [invertase]. Formasanes emerging through the reducing effect of enzymatic hydrolysate upon 2, 3, 5-triphenyltetraxoliumchloride reagent undergo elution from the chromatograph and are then identified by means of colorimetric methods.
(In Czech, English summary only)

Keywords: determination, saccharose, raffinose, reducing sugars

Published: June 1, 1963