Kvasny Prum. 1974; 20(3): 49-51 | DOI: 10.18832/kp1974008

A New culture medium for ascertaining the presence and identification of lactobacilli.Peer-reviewed article

J. ŠAVEL, M. PROKOPOVÁ

The article deals with a new culture medium which has been developed for ascertaining the presence and determination of lactobacilli. It consists of fermented wort, casein hydrolysate and a substance stimulating the propagation of lactobacilli. Two modifications of the medium, viz. one containing beta-phenylethanol the other without it were compared with two sorts of media manufactured by Sartorius Membranfilter, viz. VLB L 41 and VLB S 7. Beside normal cultures, applied in industry, several strains of pure cultures were used for inoculation. It has been found that the new medium permits to ascertain and identify lactobacilli and pediococci practically as reliably as the mentioned media VLB L 41 and VLB S7. Inmedium without b-phenylethanol propagation was more vigorous than in the medium with this substance. The authors analyze the reason of diference.
(In Czech, English summary only)

Keywords: lactobacilli, presence, determination

Published: March 1, 1974