Kvasny Prum. 2005; 51(7-8): 235-239 | DOI: 10.18832/kp2005012

Low-Alcoholic Beer Production Using Mutant Brewing Yeast.Peer-reviewed article

Radoslav SELECKÝ, Daniela ©MOGROVIČOVÁ, Martin ©ULÁK
Katedra biochemickej technológie, Fakulta chemickej a potravinárskej technológie, Slovenská technická univerzita

Possibility of low-alcoholic beer production by brewing yeast mutant in genes corresponding to tricarboxylic acid cycle enzymes was investigated. Four collection strains of Saccharomyces cerevisiae and four strains of brewing yeast isolated from Slovak breweries was exposed to UV radiation. As a result we have obtained 16 mutant yeast, ten of them were able to produce required organic acids. After fermentation of 11 % w/w. wort the final young beers showed alcohol content between 0.54-0.64 % v/v., pH varied between 4.2-4.7. Other parameters did not differ too much from those of classical lowalcoholic beers.Possibility of low-alcoholic beer production by brewing yeast mutant in genes corresponding to tricarboxylic acid cycle enzymes was investigated. Four collection strains of Saccharomyces cerevisiae and four strains of brewing yeast isolated from Slovak breweries was exposed to UV radiation. As a result we have obtained 16 mutant yeast, ten of them were able to produce required organic acids. After fermentation of 11 % w/w. wort the final young beers showed alcohol content between 0.54-0.64 % v/v., pH varied between 4.2-4.7. Other parameters did not differ too much from those of classical lowalcoholic beers.

Keywords: brewing yeast, UV mutagenesis, tricarboxylic acid cycle, low-alcoholic beer

Published: July 1, 2005 

References

  1. Zufall, C., Wackerbauer, K.: Die Entalkoholisierung von Bier durch Dialyse - Verfahrenstechnische Beeinflussung der Bierqalität und ihr Einfluss auf die Bierqalität. Mschr. Brauwiss. 53, 2000, 104-179.
  2. Adachi, E., Torigoe, M., Sugiyama, M., Nikawa, J., Shimizu, K.: Modification of methabolic pathway of S. cerevisiae by the expresion of lactate-dehydrogenase and deletion of pyruvate decarboxylase genes for the lactic acid fermentation at low pH value. J. Ferment. Bioeng. 86, 1998, 284-289. Go to original source...
  3. Nevoigt, E., Pilger, R., Mast-Gerlach, E., Schmidt, U., Freihammer, S., Eschenbrenner, M., Garbe, L., Stahl, U.: Genetic engineering of brewing yeast to reduce the content of ethanol in beer. FEMS Yeast Res. 2, 2002, 225-232. Go to original source...
  4. Perpete, E., Collin, S.: Contribution of 3-methyl thiopropionaldehyde to the worty flavour of alcohol-free beers. J. Agr. Food Chem. 47, 1999, 2374-2378. Go to original source...
  5. MacKenzie, D.: Foul Brew. New Sci. 162, 1999, 2190-2197.
  6. Kaclíková, E.: Tvorba extracelulárnych organických kyselín mutantami kvasiniek Saccharomyces cerevisiae. Bulletin potravinárskeho výskumu 3, 1996, 135-144.
  7. Mockovčiaková, D., Janitorová, V., Zigová, M., Kaclíková, E., Zagulski, M., ©ubík, J.:The ogd1 and kgd1mutants lacking 2-oxoglutarate dehydrogenase activity in yeast are allelic and can be differentiated by the cloned amber suppresor. Curr. Genet. 24, 1993, 377-381. Go to original source...
  8. Przybyla-Zawislak, B., Gadde, D. M., Ducharme, K., McCammon, M. T.: Genetic and Biochemical Interactions Involving Tricarboxylic Acid Cycle (TCA) Function Using a Collection of Mutants Defective in All TCA Cycle Genes. Genet. 152, 1999, 153-166. Go to original source...
  9. Navrátil, M., Dömény, Z., ©turdík, E., ©mogrovičová, D., Gemeiner, P.: Production of non-alcoholic beer using free and immobilized cells of Saccharomyces cerevisiae deficient in the tricarboxylic acid cycle. Biotechnol. Appl. Biochem. 35, 2002, 133-140. Go to original source...
  10. Basařová, G., Čepička, J., Doleľalová, A., Kahler, M., Kubíček, J., Poledníková, M., Voborský, B.: Pivovarsko-sladařská analytika 1-3 zv., Merkanta, Praha, 1993, 965.
  11. Back W., Lommi H., Swinkels W. J., Vilijava T. T.: European Patent No. 0 601 362 AI, 1993.
  12. Whiting, G. C.: Organic acid metabolism of yeasts during the fermentation of alcoholic bewerages - a review. J. Inst. Brew. 82, 1976, 84-92. Go to original source...