Kvasny Prumysl, 2003 (vol. 49), issue 9
Effective and easy isolation of iso-alpha acids from beer by SPE method.Peer-reviewed article
Marie JURKOVÁ, Vladimír KELLNER, Jiří ČULÍK, Tomáš HORÁK, Pavel ČEJKA
Kvasny Prum. 2003; 49(9): 258-259 | DOI: 10.18832/kp2003016 
While the analytical public agreed on the chromatographic method for determination of iso-alpha acids in beer (the EBC 7.8. method is used in international trials) there is no obligatory method for extraction these compounds and every laboratory uses its own procedure. Beside the classical liquid-liquid extraction many different ways are used to isolate target compounds, especially the SPE. Unlike the reverse phases used based on modified silicagel, a new polymeric sorbent STRATA X exceeds by higher capacity and versatility has been tried. It is applicable to a wide spectrum of compounds: acidic, basic and neutral. The preparing of the samples to analyse...
Impact of Yeast Handling on Brewing Yeast Quality.Peer-reviewed article
Jan NOVÁK, Gabriela BASAŘOVÁ, Jaromír FIALA
Kvasny Prum. 2003; 49(9): 260-263 | DOI: 10.18832/kp2003017
In this study was monitored impact of collected yeast washing intenzity and storage condition on its quality.We worked with three lager brewing yeast strains No. 2, 7, 95 (RIBM 655 - Culture Collection of Brewing Yeasts) with different genetic properties. Yeast viability was determined partly by methylene blue staining, partly by propidium iodide staining with flow cytometric analysis. Both staning methods were compared. By five times yeast washing in tap water near 4 °C were observed increased values of vitality determined by acidification power test. Measure impurity of yeast was determined by flow cytometric analysis and formulate like ratio of...