Kvasny Prum. 1983; 29(5): 98-102 | DOI: 10.18832/kp1983018

Immunochemical determination of yeasts' proteases in beer.Peer-reviewed article

I. FUKAL, A. KOWALCZYKOVÁ, J. KÁŠ

Experimental work is focused on a determination of intracellular proteases in yeasts. An extract of brewing yeast of isolated at first. This extract was divided into proteolytic fractions by gel ion-exchange chromatography. The proteolytic fractions were used for the immunization of rabbits. The antiserums obtained were used for a distinction of antigens in the fractions of yeast autolysate and for a determination of intracellular antigenes of yeasts in beer. Immunochemical methods such as immunoelectrophoresis, two- dimensional immunoelectrophoresis, double diffusion, and single radial diffusion for the distinction of antigens were applied. At the end, one example is discussed: The beer without enzymatic stabilization showed the proteolytic activity despite of the negative immunochemical determination. The results proved that the presence of yeast proteases in beer is not usual.
(In Czech, English summary only)

Keywords: Immunochemical determination, cell, yeasts

Published: May 1, 1983