Kvasny Prum. 1993; 39(4): 102-106 | DOI: 10.18832/kp1993008

Analysis of alimentary dyes from fresh-water algae by HPLC method.Peer-reviewed article

J. KOPECKÝ, M. KAJAN, V. TICHÝ

The method of the qualitative and quantitative analysis of alimentary dyes in a biomass of the fresh-water algae Chlorella vulgaris is described. The method permits the determination of chlorophylls including their degradation products, xanthophylls and carotenoids using the gradient HPLC on the reverse phase. An identification of the individual pigments and their degradation products was performed by a comparison of the spectrum obtained with the detector of diode field with the data published in a literature. Further, the effect of extraction conditions on the quality of analysis and the formation of pigment degradation products is discussed.
(In Czech, English summary only)

Keywords: alimentary dyes, algae, extraction, Chlorella vulgarit, HPLC

Published: April 1, 1993