Kvasny Prum. 1993; 39(6): 166-169 | DOI: 10.18832/kp1993012

Practical application of the reaction of fermenting hopped wort with m-phenylendiamine.Peer-reviewed article

J. ŠAVEL, M. PROKOPOVÁ, D. ZDVIHALOVÁ

The test with m-phenylendiamine (m-FDA) is used for an investigation of the formation of nitrites during a fermentation. m-FDA is added into the inoculated hopped wort. During the fermentation of contaminated worts it reacts with nitrite formed and partially with some compounds of wort, too. The reaction of m-FDA with saccharides, phenols and other compounds such as ascorbic acid was proved. The m-FDA test should be performed together with the addition of an antibiotic, e.g. tetracycline hydrochloride. The quantity of the nitrite formation is given by the change of absorbance of both samples at 450 nm. This quick screening test is proper for a preliminary identification of fermented worts containing higher levels of nonvolatile nitrosamines (ATNC).
(In Czech, English summary only)

Keywords: mashed wort, m-phenylendiamine, nitrates

Published: June 1, 1993