Kvasny Prum. 2015; 61(2): 46-50 | DOI: 10.18832/kp2015007

Analytical strategies for the determination of deoxynivalenol and its modified forms in beer: A mini review.Peer-reviewed article

Alexandra MALACHOVÁ, Elisabeth VARGA, Heidi Elisabeth SCHWARTZ-ZIMMERMANN, Franz BERTHILLER
Christian Doppler Laboratory for Mycotoxin Metabolism and Center for Analytical Chemistry, Department for Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Konrad Lorenz Str. 20, 3430 Tulln, Austria

The aim of this review is to provide a brief overview of analytical methods used for the determination of deoxynivalenol and its modified forms deoxynivalenol-3-β-D-glucoside, 3-acetyl-deoxynivalenol and 15-acetyl-deoxynivalenol in beer. The analytical methods discussed involve gas chromatography coupled with flame ionization detection, electron capture detection and mass spectrometry as well as liquid chromatography hyphenated to ultra-violet detection and mass spectrometry. Special attention was paid to sample preparation. Immunochemical methods such as enzyme-linked immunosorbent assays (ELISAs) which represent efficient tools for fast screening of beer with no sample purification are also discussed
(In English).

Keywords: DON, deoxynivalenol-3-glucoside, masked mycotoxins, HPLC-MS, GC-MS, ELISA

Received: December 6, 2014; Accepted: January 19, 2015; Published: February 1, 2015 

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