Kvasny Prum. 1958; 4(2): 25-29 | DOI: 10.18832/kp1958003

Classification of alpha- and beta- amylase in malting process by means of chromatographic method.Peer-reviewed article

E. ŠTEFCANOVÁ-BOLFOVÁ

The author comes to conclusion, that under the given experimental conditions the amylolytic enzyme forms two bots, representing alpha and beta-amylase respectively. It was ascertained, that at the beginning of malting the amylases are blocked by the proteins and their motion is changed or even oppressed. First the growth of proteolytic activity, which is characterized by the presence of 115 to 150 mg of amine nitrogen in100 gof dry matter of malt makes the chromatographic separation of enzymes possible.The malting on the malt-floor brings the best conditions for activation of enzymes. The first of maximal proteolytic activity of enzymes appeared after two days, the optimal activity of alpha amylase after 3 1/2 days and the chromatographic separation of both amylases after four days. The drum malt showed the least diastatic ability. The first maximum of proteolytic activity appeared after 3 3/4 days, the optimal activity of alpha-amylase after 6 days. The separation on the paper took place after five or six days.
(In Slovak, English summary only)

Keywords: alpha-amylases, beta-amylases, paper chromatography

Published: February 1, 1958