Kvasny Prum. 1976; 22(10): 232-233 | DOI: 10.18832/kp1976033

Application of cappilary gas chromatography for the determination of volatile fatty acids present in molasses.Peer-reviewed article

J. HRIVŇÁK, M. MEDVEĎ

The method developed by the authors for selective determination of lower fatty acids present in molasses is based on the applications of gas chromatography. The sequence of operations is as follows: distilling of acids from molasses into an alkaline medium, condensation, acidulation (1 : 1 H₂SO₄). Extraction by ethyl ether and concentration of extract. Concentrated extract can be analyzed by gas chromatography in a capillary column 45 m long with internal diameter of 0.2 mm. The column is made of stainless steel and wetted with polypropylénglycol (UCON LB 550X + H₃PO₄). The column is kept at 140°C, the injection chamber at 190°C, the pressure of nitrogen at inlet end of the column being 1.0 kp/cm² . Molasses contain a number of various acids, viz.: acetic, propionic, i-butyric, n-methylbutyric, (±)- 2-methylbutyric, n - valeric and n- hexanoic.
(In Slovak, English summary only)

Keywords: molasses, volatile fatty acids, gas chromatography

Published: October 1, 1976