Kvasny Prum. 1977; 23(7): 150-152 | DOI: 10.18832/kp1977025

Application of the Kunitake-Kobayshi method for the determination of proteins in malt and wort.Peer-reviewed article

A. DOLEŽALOVÁ, M. NENTWICHOVÁ

The authoresses have already published in Kvasný průmysl the results of their research works into the determination of proteins in barley. The present article deals with the application of the Kunitake-Kobayshi method for the determination of proteins also in malt and wort. The principle of the method was strictly adhered to, though the size of samples was adjuste (2,2 g of malt, 55 ml of wort taken with a pipette). Errors in the obtained data do not exceed those typical for the Kjeldahl method and the reproducibility is practically the same as with the Kjel-Fosse instruments. The Kunitake-Kobayshi method can be used to advantage for the determination of proteins in malt aod of dissolved nitrogen in wort, since it is simple, saves much time, gives accurate results and is reproducible. It can be therefore recommended to all laboratories in brewing and malting industries.
(In Czech, English summary only)

Keywords: malt, wort, proteins, Kunitake-Kobayshi method

Published: July 1, 1977