Kvasny Prum. 1981; 27(10): 232-235 | DOI: 10.18832/kp1981044

The production and characteristics of microbial cellulases.Peer-reviewed article

M. GOTTVALDOVÁ

The results are reviewed of the study of cellulases which has been carried out at the Research Institute of Food Industry in Prague during recent years. By means of selection of strains producing cellulases from natural sources and their mutations, a productive strain of Trichoderma viride has been obtained. For the optimum enzyme formation, cellulose as the source of carbon and inducer, an inorganic source of nitrogen, and a relatively high concentration of phosphate are reguired. The employment of such carbon sources (derivatives of cellulose) which enable higher specific growth rates results in a decrease in the enzym production. On the other hand, an improvement of the contact between liquid and the insoluble carbon source, which can be achieved by surface active agents, results in an increase in the enzyme production. Several enzyme fractions have been prepared by ionex chromatography but none of them, has revealed homogenenity by electrophoretical methods.
(In Czech, English summary only)

Keywords: microbial cellulases, production

Published: October 1, 1981