Kvasny Prum. 1983; 29(6): 130-134 | DOI: 10.18832/kp1983024

Lytic enzymes and the possibilities of their application.Peer-reviewed article

E. GALAS, S. BIELECKI, T. ANTCZAK

Numerous methods such as autolysis, chemical and mechanical treatments, howe been used for yeast cell disruption, especially in connection with the use of single cell proteins as a human food. The major disadvantages of these methods (long reaction time, potential toxicity and high cost) are considerably eliminated by enzymatic lysis of cell walls. In this connections, this paper describes studies on the biodegradation of yeast cell walls caused by enzymes secreted into culture of Streptomyces sp. 1228. The major components of this enzymatic system are two b-1,3-glucanases, b-1,6-glucanase, chitase and peptide hydrolase. The release of intracellular proteins and carbohydrates was followed in relation to the influence of pH and time of incubation in relation to the influence of pH and time of incubation of cells with lytic system. Further research was directed to the application of immobilized lytic system.
(In Czech, English summary only)

Keywords: lytic enzymes, application

Published: June 1, 1983