Kvasny Prum. 1984; 30(4): 82-88 | DOI: 10.18832/kp1984017

Comparison of physiological characteristics of cells in batch and continuous cultures.Peer-reviewed article

J. PÁCA

The effect of oxygen supply on the changes in total and specific rate of oxygen consumption by cells, oxygen transfer rate, saturation concentration of dissolved oxygen and the yields of batch and continuous cultivation was studied. The microorganism used was Klebsiella aerogenes CCM 2318 growing in synthetic glucose medium. Continuous cultures were carried out at dilution rates of 0.96 and 0.178 h^-1. The rate of oxygen transfer was determined by the sulphite methods and the coefficient K_La was assessed using the dynamic method with a correction for changes in the saturation of dissolved oxygen. A decreased oxygen supply in batch cultures caused deformation in the course of cell respiration. Comparison of results batch and continuous cultivations shower that the highest yields Y x/sand Y x/o₂ are attained at low dilution rates without oxygen limitation. Batch cultures, on the other hand, exhibit the lowest yields and the highest cell respiration levels. In both types of cultivations, a respiration peak was ascertained under conditions of growth limitation by oxygen.
(In Czech, English summary only)

Keywords: microorganisms, cultivation, oxygen, Klebsiella aerogenes

Published: April 1, 1984