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Kvasny Prum. 1985; 31(3): 58-60 | DOI: 10.18832/kp1985010
A determination of anthocyanidins and anthocyanins in addition to other natural and synthetic dyestuffs in fruit and vegetable beverages is described. The first step of the determination comprises an extraction of dyestuffs using a suitable sorbens, e.g. polyvinylpyrrolidone, polyamide or Dowex resin. Second step is the separation of anthocyans by the TLC or column chromatography using microcrystallinic cellulose, silica gel, polyvinylpyrrolidone and Spheron S 1000. Isolated anthocyans are verified by selective chemical reactions. The quantity determination of anthocyans after their chromatographic separation is made by the colorimetry. Empirical formulae are used for the calculation of their concentrations.
(In Czech, English summary only)
Published: March 1, 1985