Kvasny Prum. 1986; 32(1): 17-20 | DOI: 10.18832/kp1986005

Characterization of non-alcoholic beverages using microextraction and GC.Peer-reviewed article

J. HRIVŇÁK, J. ILAVSKÝ, J. TAKÁČOVÁ

In the first part the methods of isolation of organic compounds from non-alcoholic beverages including the possibilitics of their estimation by the GC with the capillary column are discussed. In the experimental part microextraction for the isolation was used. The extract was analysed using the GC in the capillary column. 250 ml of the beverage cooled to +5 °Cwas extracted by pentane (0.5 ml). The separation of the thin layer of the extraction agent was performed in the special apparatus according to Czechoslovak Patent No. 215 680. The extract (1.5 ml) was directly analyzed using injection technique in a glass capillary column with a length of45 mand with an inner diameter of0.3 mm. The column was treated with the silicon phase OV-101. The temperature of the column during a sampling was40 °Cthen was increased according to the program to210 °Cat a rate of4 °Cper minute. Further, a schema of the apparatus for a separation of the thin layer of extraction agent is shown. The result in the form of chromatograms of beverages Pepsi-Cola, Kofola, orange juice and lemon juice are discussed. The possible quantitative analysis of the results is discussed on an example of the direct analysis of arrange concentrate and after its isolation from a water solution. The advantages of microextraction are as follows: an achievement of high concentration, a possibility of the direct analysis of the extract without its further concentration, a relatively short time of isolation, a simple apparatus and an economic suitability of microextraction due to low consumption of he extraction agent.
(In Slovak, English summary only)

Keywords: non-alcoholic beverages, aroma, micorextraction, gas chromatography

Published: January 1, 1986