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Kvasny Prum. 1989; 35(3): 74-78 | DOI: 10.18832/kp1989011
A method of the complete yeast cell fractionation has been developed. After the yeast cell disintegration using the process of the initiated autolyse (performed under the optimum conditions for the release of the cytoplasmatic content as well as with the aim of an isolation the following fractions) can be obtained: the yeast extract with 70 to 80 % proteins and their degradation products having god sensorial properties (taste, colour, fragrance) and powder consistency, the preparate of invertase (tasteless while powder) with the enzyme activity of 10 mkat per g dry weight, the phospholipids fraction (paste of dark brown colour) containing 30 % of the active compound, ergosterol (in the form of white-yellow crystals) with the purity above 62 %, cell walls (with or without lipids) containing more than 70 % of polysaccharides.
(In Slovak, English summary only)
Published: March 1, 1989