Kvasny Prum. 2001; 47(6): 159-163 | DOI: 10.18832/kp2001013

Proteomics - a new and fast tool for identification of barley varieties.Peer-reviewed article

J. CHMELÍK, P. ŘEHULKA, C. MAYRHOFER, G. ALLMAIER

Identification of several proteins from various barley samples by the proteomic method based on combination of gel electrophoresis, MALDI-TOF mass spectrometry, and bioinformatics is an important tool for proteome analysis of barley and it can be successfully applied to the identification of malting barley varieties. The mass spectrometry-based strategy designed for protein identification exhibits good sensitivity and rapid protein identification in comparison to other methods. The identified proteins were ribulose bishophate carboxylase from barley leaves and beta-amylase and aldose-reductase from barley grainsIdentification of several proteins from various barley samples by the proteomic method based on combination of gel electrophoresis, MALDI-TOF mass spectrometry, and bioinformatics is an important tool for proteome analysis of barley and it can be successfully applied to the identification of malting barley varieties. The mass spectrometry-based strategy designed for protein identification exhibits good sensitivity and rapid protein identification in comparison to other methods. The identified proteins were ribulose bishophate carboxylase from barley leaves and beta-amylase and aldose-reductase from barley grains

Keywords: proteomics, protein identification, barley, variety identification, mass spectrometry

Published: June 1, 2001