Kvasny Prum. 2007; 53(7-8): 206-214 | DOI: 10.18832/kp2007012

Brewing and the taxonomy of brewer's yeast.Peer-reviewed article

Dagmar MATOULKOVÁ, Jan ŠAVEL
1 VÚPS, a. s., Pivovarský ústav Praha
2 Budějovický Budvar, n.p., České Budějovice

The review deals with the history, methods and current state of brewer's yeast taxonomy. An important factor in ensuring standard quality of finished beer is the use of pure yeast cultures in the production.Yeast used to be classified on the basis of its phenotype by the methods of traditional taxonomy, which are briefly addressed in the text. A short overview is given of the history of brewer's yeast classification and nomenclature. Particular attention is paid to molecular biological techniques that enable us to classify yeast on the basis of its phylogenetic relatedness. Basic principles of selected molecular biological methods and their use in brewer's yeast taxonomy are explained.We recommend restoring the historical name Saccharomyces carlsbergensis for bottom yeast and using Saccharomyces cerevisiae for top brewer's yeast.The review deals with the history, methods and current state of brewer's yeast taxonomy. An important factor in ensuring standard quality of finished beer is the use of pure yeast cultures in the production.Yeast used to be classified on the basis of its phenotype by the methods of traditional taxonomy, which are briefly addressed in the text. A short overview is given of the history of brewer's yeast classification and nomenclature. Particular attention is paid to molecular biological techniques that enable us to classify yeast on the basis of its phylogenetic relatedness. Basic principles of selected molecular biological methods and their use in brewer's yeast taxonomy are explained.We recommend restoring the historical name Saccharomyces carlsbergensis for bottom yeast and using Saccharomyces cerevisiae for top brewer's yeast.

Keywords: taxonomy, brewing industry, yeast, history, PCR, DNA

Published: July 1, 2007 

References

  1. Bamforth, C.W.: Rising to the challenge. Brewers' Guard. 132, 2003, 24-27.
  2. Anderson, R.G.: Louis Pasteur (1882-1895): An assesment of his impact on the brewing industry. Proc. Eur. Brew. Conv. Brussels, 1995, Contr. 2, 13-23.
  3. Basařová, G.:Hlaváček, I.:České pivo.2.vyd., Nuga, Pacov, 1999.
  4. Jackson, M.: Great beer guide. 1st. ed., Dorling Kindersley, London, 2000.
  5. Šavel, J.: Jak nazývat pivovarské kvasinky. Pivovarský kalendář 1998, VÚPS, Praha, 1997, 106-112.
  6. Barnett J.A., Payne R.W., Yarrow D.: Yeasts: Characteristics and identification. 3rd edition. Cambridge University Press, Cambridge, England, 2000.
  7. Kocková-Kratochvílová A.Taxonómia kvasiniek a kvasinkovitých mikroorganizmov. 1.vyd., ALFA, Bratislava, 1990.
  8. Vaughan-Martini A.: Reflections on the classification of yeasts for different end-users in biotechnology, ecology, and medicine. Int. Microbiol. 6, 2003, 175-182. Go to original source...
  9. Rainieri S., Zambonelli C., Kaneko Y.: Saccharomyces sensu stricto: Systematics, genetics diversity and evolution. J. Biosci. Bioeng. 96, 2003, 1-9. Go to original source...
  10. Možina S.S., Dlauchy D., Deak T., Raspor P.: Identification of Saccharomyces sensu stricto and Torulaspora yeasts by PCR ribotyping. Lett. Appl. Microbiol. 24, 1997, 311-315. Go to original source...
  11. Barnett J.A: A history of research on yeasts 8: taxonomy. Yeast 21, 2004, 1141-1193. Go to original source...
  12. Campbell I.: Systematics of yeasts. Brewing Microbiology. (eds Priest F.G., Campbell I.), 1st edition, Elsevier Applied Science Publishers, England, 1987. Go to original source...
  13. Lodder J., Kreger van-Rij N.J.W.: The yeasts, a taxonomic study. 1st edition, North Holland Publishing Company, Amsterdam, 1952.
  14. Lodder J. (ed.): The yeasts,a taxonomic study. 2nd edition, North Holland Publishing Company, Amsterdam, Netherlands, 1970.
  15. Kreger-van Rij N.J.W. (ed.): The yeasts, a taxonomic study. 3rd edition, Elsevier-North-Holland, Amsterdam, Netherlands, 1984.
  16. Fernández-Espinar M.T., Barrio E., Querol A.: Analysis of the genetic variability in the species of the Saccharomyces sensu stricto complex. Yeast 20, 2003, 1213-1226. Go to original source...
  17. Boulton C., Quain D.: Brewing yeast and fermentation. 1st edition, Blackwell Science Ltd., London, England, 2001.
  18. Casaregola S., Hguyen H.-V., Lapathitis G., Kotyk A., Gaillardin C.: Analysis of the constitution of the beer yeast genome by PCR, sequencing and subtelomeric sequence hybridization. Int. J. Syst. Evol. Microbiol. 51, 2001, 1607-1618. Go to original source...
  19. Edwards-Ingram L.C., Gent M.E., Hoyle D.C., Hayes A., Stateva L.I., Oliver S.G.:Comparative genomic hybridization provides new insights into the molecular taxonomy of the Saccharomyces sensu stricto complex. Genome Res. 14, 2004, 1043-1051. Go to original source...
  20. James S.A., Cai J., Roberts I.N., Collins M.D.:A phylogenetic analysis of the genus Saccharomyces based on 18S rRNA gene sequences: Description of Saccharomyces kunashirensis sp. nov. and Saccharomyces martiniae sp. nov. Int. J. Syst. Bacteriol. 47, 1997, 453-460. Go to original source...
  21. Alberts B., Bray D., Johnson A., Lewis J., Raff M., Roberts K., Walter P.: Základy buněčné biologie. 1. vyd., Espero Publishing, Ústí nad Labem, 1998.
  22. de Barros Lopes M., Soden A., Martens A.L., Henschke P.A., Langridge P.: Differentiation and species identification of yeasts using PCR. Int. J. Syst. Bacteriol. 48, 1998, 279-286. Go to original source...
  23. Piškur J., Langkjèr R.B.:Yeast genome sequencing: the power of comparative genomics. Mol. Microbiol. 53, 2004, 381-389. Go to original source...
  24. Tornai-Lehoczki J., Dlauchy D.:Delimination of brewing yeast strains using different molecular techniques. Int. J. Food Microbiol. 62, 2000, 37-45. Go to original source...
  25. Montrocher R., Verner M.C., Briolay J., Gautier C., Marmeisse R.: Phylogenetic analysis of the Saccharomyces cerevisiae group based on polymorphisms of rDNA spacer sequences. Int. J. Syst. Bacteriol. 48, 1998, 295-303. Go to original source...
  26. Azumi M., Goto-Yamamoto N.: AFLP analysis of type strains and laboratory and industrial strains of Saccharomyces sensu stricto and its application to phenetic clustering. Yeast 18, 2001, 1145-1154. Go to original source...
  27. Kurtzman C.P., Robnett C.J.:Phylogenetic relationship among yeasts of the Saccharomyces complex determined from multigene sequence analyses. FEMS Yeast Res. 3, 2003, 417-432. Go to original source...
  28. Kurtzman C.P.: Phylogenetic circumscription of Saccharomyces, Kluyveromyces and other members of the Saccharomycetaceae, and the proposal of the new genera Lachancea, Nakaseomyces, Naumovia, Vanderwaltozyma and Zygotorulaspora. FEMS Yeast Res. 4, 2003, 233-245. Go to original source...
  29. Laidlaw L., Tompkins T.A., Savard L., Dowhanick T.M.: Identification and differentiation of brewing yeasts using specific and RAPD polymerase chain reaction.J.Am.Soc.Brew.Chem.54, 1996, 97-102. Go to original source...
  30. Barszczewski W., Robak M.: PCR-based differentiation and homology of brewing and type strains of the genus Saccharomyces. J. Inst. Brew. 112, 2006, 165-172. Go to original source...
  31. Gomes L.H., Duarte K.M.R., Argueso J.L., Echeverrigaray S., Tavares F.C.A.: Methods for yeast characterization from industrial products. Food Microbiol. 17, 2000, 217-223. Go to original source...
  32. Piškur J., Smole Možina S., Stenderup J., Pedersen M.B.: A mitochodrial molecular marker, ori-rep-tra, for differentiation of yeast species. Appl. Environ. Mirobiol. 61, 1995, 2780-2782. Go to original source...
  33. Esteve-Zarzoso B., Belloch C., Uruburu F., Querol A.: Identification of yeasts by RFLP analysis of the 5,8S rRNA gene and the two ribosomal internal transcribed spacer. Int. J. Syst. Bacteriol. 49, 1999, 329-337. Go to original source...
  34. Perpéte P., Van Cutsem P., Boutte C., Colson-Corbisier A.-M., Collin S.: Amplified fragment-length polymorphism, a new method for the analysis of brewer's yeast DNA polymorphism. J. Am. Soc. Brew. Chem. 59, 2001, 195-200. Go to original source...
  35. de Barros Lopes M., Rainieri S., Henschke P.A., Langridge P.: AFLP fingerprinting for analysis of yeast genetic variation. Int. J. Syst. Bacteriol. 49, 1999, 915-924. Go to original source...
  36. Quain D.E. Yeast genetics in brewing: new insights and opportunities. Brewing: New technologies (ed. Bamforth C.W.), 1st edition, Woodhead Publishing Limited, Cambridge, England. 2006. Go to original source...
  37. Polaina J., Brewer's yeast: genetics and biotechnology. Appl. Mycol. Biotechnol. 2, 2002, 1-17. Go to original source...
  38. Naumov G.I., James S.A., Naumova E.S., Louis E.J., Roberts I.N.:Three new species in the Saccharomyces sensu stricto complex: Saccharomyces cariocanus, Saccharomyces kudriavzevii and Saccharomyces mikatae. Int. J. Syst. Evol. Microbiol. 50, 2000, 1931-1942. Go to original source...
  39. Rainieri S., Kodama Y., Kaneko Y., Mikata K., Nakao Y., Ashikari T.: Pure and mixed lines of Saccharomyces bayanus and Saccharomyces pastorianus and their contribution to the lager brewing strain genome. Appl. Environ. Microbiol. 72, 2006, 3968-3974. Go to original source...
  40. Ramos J.P., Valente P., de Souza R.A., Rosa C.A., Leoncini O.: Heteroduplex mobility assay of the D1/D2 region of the 26S rDNA for differentiation of Saccharomyces species. Lett. Appl. Microbiol. 33, 2001, 206-210. Go to original source...
  41. Sato M., Kishimoto M., Watari J., Takashio M.: Breeding of brewer's yeast by hybridization between a top-fermenting yeast Saccharomyces cerevisiae and a cryophilic yeast Saccharomyces bayanus. J. Biosci. Bioeng. 93, 2002, 509-511. Go to original source...
  42. Querol A., Belloch C., Fernandez-Espinar M.T., Barrio E.: Molecular evolution in yeast of biotechnological interest. Int. Microbiol. 6, 2003, 201-205. Go to original source...
  43. Storgards E., Haikara A., Juvonen R.: Brewing control systems: microbiological analysis.Brewing:New technologies.(ed.by Bamforth C.W.), 1st edition, Woodhead Publishing Limited, Cambridge, England, 2006. Go to original source...
  44. Steenberg, J., Gubiš, J., Melicharová, E., Šimek, V., Goldmann, R.: Dynamický sběr kvasnic z CKT a jejich asimilace před zakvašováním - kvasničné hospodářství Gambrinus. Kvasny Prum. 49, 2003, 30-34. Go to original source...
  45. Bendová, O., Kahler, M: Pivovarské kvasinky. 1.vyd., SNTL, Praha, 1981.
  46. Košin, P., Šavel, J., Kolouchová, I., Brož, A.: Viabilita a vitalita kvasnic v provozním kvašení. Kvasny Prum. 53, 2007, 30-34. Go to original source...
  47. Čapková, V., Janík, P., Potěšil, V.: Restaurační minipivovary v České republice. 1.vyd., VÚPS, Praha, 1999.
  48. Šuráň, J.: Pšenice a pivo. Pivař 2006 (1; 7/8), 17-18.
  49. Narziss, L., Miedaner, H., Nitzsche, F.: Ein Beitrag zur Bildung von 4-Vinyl-Guajakol bei der Herstellung von bayerische Weizenbier. Monatsschr. Brauwiss. 43, 1990, 96-100.
  50. Hlaváček, F. A., a kol.: Pivovarské kvasnice. 1. vyd., SNTL, Praha, 1958.
  51. Kurtzman C.P., Fell J.W. (eds.): The yeasts, a taxonomic study. 4th edition. Elsevier, Amsterdam, 1998.