Kvasny Prum. 2010; 56(11-12): 428-432 | DOI: 10.18832/kp2010044

Determination of Trans-2-Nonenal in Barley Grain, Malt and Beer.Peer-reviewed article

Zdeněk SVOBODA, Renata MIKULÍKOVÁ, Sylvie BĚLÁKOVÁ, Karolína BENEŠOVÁ, Ivana MÁROVÁ, Zdeněk NESVADBA
1 VÚPS, a. s., Sladařský ústav Brno, Mostecká 7, 614 00 Brno
2 FCH VUT v Brně, Purkyňova 118, 612 00 Brno
3 Agrotest Fyto, s. r. o., Havlíčkova 2787/121, 768 01 Kroměříž

Trans-2-nonenal is an aldehyde contributing to an unpleasant off-flavor and odor of rancid butter in stored beer. The aim of this study was to optimize and introduce automated solid phase microextraction technique coupled with gas chromatography for determination of trans-2-nonenal in beer and brewing materials. Five types of SPME fibers coated with different stationary phases (100 μm PDMS, 65 μm PDMS/DVB, 85 μm CAR/PDMS, 50/30 μm DVB/CAR/PDMS, 85 μm PA) were tested for their efficiencies in the headspace SPME. The highest HS-SPME extraction efficiency was achieved with the PDMS/DVB fiber and addition of 1.5 g of NaCl, extraction time was 20 minutes at 60 °C. Trans-2-nonenal was identified with the method of HS-SPME-GC-MS; the samples were analyzed using the HS-SPMEGC- FID technique.Trans-2-nonenal is an aldehyde contributing to an unpleasant off-flavor and odor of rancid butter in stored beer. The aim of this study was to optimize and introduce automated solid phase microextraction technique coupled with gas chromatography for determination of trans-2-nonenal in beer and brewing materials. Five types of SPME fibers coated with different stationary phases (100 μm PDMS, 65 μm PDMS/DVB, 85 μm CAR/PDMS, 50/30 μm DVB/CAR/PDMS, 85 μm PA) were tested for their efficiencies in the headspace SPME. The highest HS-SPME extraction efficiency was achieved with the PDMS/DVB fiber and addition of 1.5 g of NaCl, extraction time was 20 minutes at 60 °C. Trans-2-nonenal was identified with the method of HS-SPME-GC-MS; the samples were analyzed using the HS-SPMEGC- FID technique.

Keywords: barley, beer, gas chromatography, malt, SPME, trans-2- nonenal

Received: September 6, 2010; Accepted: October 7, 2010; Published: November 1, 2010 

References

  1. Angelo, St., A. J., Ory, L. R.: Lipid Degradation During Seed Deterioration, Symposium: Deterioration Mechanisms in Seeds, Southern Regional Research Center, ARS, US Department of Agriculture, 1983. Go to original source...
  2. Velíšek, J.: Chemie potravin 1, Ossis, Tábor, 2002, 117-161.
  3. Drost, B. W., Van Berg, R., Freijee, F. J. M., Van Velde, E. G., Hollemans M.: Flavor stability. J. Am. Soc. Brew. Chem. 48, 1990, 124-131. Go to original source...
  4. Kobayashi, N., Kenada, H., Kano, Y., Koshino, S.: Determination of wort production, Proceedings of 24th Congress of the European Brewery Convention, Oslo, 1993, 405-412.
  5. Svoboda, Z., Mikulíková, R., Běláková, S., Benešová, K., Nesvadba, Z.: Stanovení obsahu lipidů a zastoupení mastných kyselin v obilkách ječmene a ve sladu, Kvasny Prum. 55, 2009, 315-320. Go to original source...
  6. Basařová, G., Šavel, J., Basař, P., Lejsek, T.: Pivovarství. VŠCHT, Praha, 2010, ISBN 97880-7080-734-7.
  7. Skadhauge, B., Knudsen, S., Lok, F., Olsen, O.: Barley for production of flavour-stable beer, Proceedings of 30th Congress of the European Brewery Convention, Prague, 2005, 676-678.
  8. Scherer, R., Wagner, R., Hoffmann Kowalski, C., Teixeira Godoy, H.: (E)-2-Nonenal determination in Brazilian beers using headspace solid-phase microextraction and gas chromatographic coupled mass spectrometry (HS-SPME-GC-MS), Ciênc. Tecnol. Aliment. 30, supl.1 Campinas, 2010, 161-165. Go to original source...
  9. Veselý, P. et al.: Analysis of aldehydes in beer using solid-phase microextraction with onfiber derivatization and gas chromatography/mass spectrometry. J. Agric. Food Chem. 51, 2003, 6941-6944. Go to original source...

Return to the content