Kvasny Prum. 2013; 59(3): 58-62 | DOI: 10.18832/kp2013006

Determination of fatty acids in beer by fast routine analyse.Peer-reviewed article

Tomáš HORÁK, Jiří ČULÍK, Marie JURKOVÁ, Pavel ČEJKA, Jana OLŠOVSKÁ
Výzkumný ústav pivovarský a sladařský, a. s., Pivovarský ústav Praha, Lípová 15, 120 44 Praha 2

Fatty acids in beer originate not only from raw materials but their content depends also on the duration of fermentation. An increased content of fatty acids during fermentation of wort can influence the quality of final beer. For this reason it is necessary to use a fast and routine analytical method for the determination of their concentration in beer or hopped wort. This paper presents a suitable procedure based on solid phase extraction (SPE). The final determination of fatty acids was carried out using faster gas chromatography with silica capillary column of 0.18 mm internal diameter. Flame ionization detector (FID) was used for the detection. Working parameters of this procedure are described in this study.Fatty acids in beer originate not only from raw materials but their content depends also on the duration of fermentation. An increased content of fatty acids during fermentation of wort can influence the quality of final beer. For this reason it is necessary to use a fast and routine analytical method for the determination of their concentration in beer or hopped wort. This paper presents a suitable procedure based on solid phase extraction (SPE). The final determination of fatty acids was carried out using faster gas chromatography with silica capillary column of 0.18 mm internal diameter. Flame ionization detector (FID) was used for the detection. Working parameters of this procedure are described in this study.

Keywords: fatty acids, beer, solid-phase extraction (SPE), faster gas chromatography

Received: December 11, 2012; Accepted: February 4, 2013; Published: March 1, 2013 

References

  1. Baltusen, E., Sandra, P., David, F., Cramers, C., 1999: Stir bar sorptive extraction (SBSE), a novel extraction technique for aqueous samples: theory and principles. J. Microcolumn Sep. 11: 737-747. Go to original source...
  2. Bicchi, C., Iori, C., Rubiolo, P., Sandra, P., 2002: Headspace sorptive extraction (HSSE), stir bar sorptive extraction (SBSE) and solid phase microextraction (SPME) applied to the analysis of roasted Arabica coffee and coffee brew. J. Agric. Food Chem. 50: 449-459. Go to original source...
  3. Clapperton, J. F., 1978: Fatty acids contributing to caprylic flavour in beer. The use of profile and threshold data in flavour reseach. J. Inst. Brew. 84: 107-112. Go to original source...
  4. Clapperton, J.F., 1978: Caprylic flavour as a feature of beer flavour. J. Inst. Brew. 84: 90-92. Go to original source...
  5. Clarke, B. J., Davine, D. F., Hawthorne, D. B., Kavanagh, T. E., Moulder, P. J., 1981: Factors affecting the formation of medium chain fatty acids during fermentation. Tech. Q. Master Brew. Assoc. Am. 18: 188-194.
  6. Dominguez, X.A., Canales, A.M., 1974: Oxidation of beer. A rational mechanism for the degradation of unsaturated fatty acids and the formation of unsaturated aldehydes. Brewers Digest 49: 40-47.
  7. Drost, B.W., Van Eerde, P., Hoekstra, S.F., Strating, J., 1971: Fatty acids and staling of beer. Proceedings of the European Brewery Convention Congress, Estoril, Elsevier Scientific: Amsterdam, The Netherlands: 451-458.
  8. Hage, T., 1987: Free fatty acids in beer - the use of a Binder-phase column in the extraction of free fatty acids for gas chromatographic essay. Proc. Fourth European Conference on Food Chemistry, Volume1, Loen, Norway, June 1-4: 106-110.
  9. Horák, T., Čulík, J., Jurková, M., Čejka, P., Kellner, V., 2005: Determination of the fatty acids in beer by SMPE. Kvasny Prum. 51: 374-377. Go to original source...
  10. Horák, T., Čulík, T., Čejka, P., Jurková, M., Kellner, V., Dvořák, J., Hašková, D., 2009: Analysis of free fatty acids in beer: Comparison of solid-phase extraction, solid-phase microextraction, and stir bar sorptive extraction. J. Agric. Food Chem. 57: 11081-11085 Go to original source...
  11. Horák, T., Čulík, J., Jurková, M., Čejka, P., Kellner, V., 2008: Determination of free medium- chain fatty acids in beer by stir bar sorptive extraction. J. Chromatogr. A,1196-1197: 96-99. Go to original source...
  12. Horák, T., Čulík, J., Jurková, M., Čejka, P., Kellner, V., Dvořák, J., Hašková, D., 2009: Faster gas chromatography and its utilization in brewing, part 1. - theoretical and practical aspects. Kvasny Prum. 55: 250-254. Go to original source...
  13. Horák, T., Čulík, J., Jurková, M., Čejka, P., Kellner, V., Dvořák, J., Hašková, D., 2009: Faster gas chromatography and its utilization in brewing, part 3. - the determination of some low volatile beer flavours. Kvasny Prum. 55: 304-309. Go to original source...
  14. Horák, T., Čulík, J., Jurková, M., Čejka, P., Kellner, V., Dvořák, J., Hašková, D., 2010: Possibilities of utilization of modern sample preparation methods for gas chromatographic analyses in beverage and namely brewing analytics. Part III. - solid- phase microextraction and stir bar sorptive extraction in fatty acids analysis in beer. Kvasny Prum. 56: 418-422. Go to original source...
  15. Chen, E. C.-H., 1980: Utilization of wort fatty acids by yeast during fermentation. J. Am. Soc. Brew. Chem. 38: 148-153. Go to original source...
  16. Literatura firmy Merck, 2000: ChromBook, 2nd Edition: 15.
  17. Literatura firmy Merck, 2005: ChromBook 2006/07: 22-23.
  18. Meilgaard, M. C., 1975: Flavor chemistry of beer. Part I: Flavor interaction between principal volatiles. Tech. Q. Master Brew. Assoc. Am. 12: 107-117.
  19. Procházková, D., 1999: Mikroextrakce tuhou fází - SMPE. Kvasny Prum. 45: 321-322.
  20. Quimby, B.D., Giarrocco, V., Klee, M.S., Hewlett-Packard Application Note 228-294, February 1995, publication number (43) 5963-5190E.
  21. Snyder, W.D., Blumberg, L., in: Sandra, P., Lee, M.L. (Eds.), Proc. 14th Int. Symp. On Capillary Chromatography, Baltimore, May 1992: 28.
  22. Tressl, R., Bahri, D., Silwar, R., 1979: Formation of aldehydes by oxidation of lipids and their importance as "off-flavour" components in beer. European Brewery Convention; IRL Press, Oxford University Press: Oxford, U.K.: 27-41.

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