Kvasny Prum. 2014; 60(9): 226-232 | DOI: 10.18832/kp2014021

An immunochemical method for the determination of Ochratoxine A in the wine and its applications.Peer-reviewed article

Marcel KARABÍN, Aneta LUČOKOVÁ, Jaromír FIALA, Lukáš JELÍNEK, Tereza HUDCOVÁ, Deliang WANG, Pavel DOSTÁLEK
1 Ústav biotechnologie, Vysoká škola chemicko-technologická v Praze, Technická 5, Praha 6, 166 28
2 China National Institute of Food and Fermentation Industries, Beijing, China

Recently, the monitoring of the occurrence of mycotoxins in raw materials used for food processing and in the final products has become one of the most important topics in food analyses. This study tests the usability of the determination of ochratoxin A based on immunochemical principles. A total of 28 samples of Czech, Portuguese and Chinese wines were analysed and the results were compared with the results obtained by means of the routinely used HPLC-FLD method. The results obtained by means of the immunochemical determination proved unequivocally to be significantly overestimated. Nevertheless, this method could be used as a simple approach for the detection of objectionable samples.Recently, the monitoring of the occurrence of mycotoxins in raw materials used for food processing and in the final products has become one of the most important topics in food analyses. This study tests the usability of the determination of ochratoxin A based on immunochemical principles. A total of 28 samples of Czech, Portuguese and Chinese wines were analysed and the results were compared with the results obtained by means of the routinely used HPLC-FLD method. The results obtained by means of the immunochemical determination proved unequivocally to be significantly overestimated. Nevertheless, this method could be used as a simple approach for the detection of objectionable samples.

Keywords: mycotoxins, wine, ochratoxin A, immunochemical determination, Ultra Performance Liquid Chromatography (UPLC)

Received: May 8, 2014; Accepted: June 28, 2014; Published: September 1, 2014 

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