Kvasny Prum. 2017; 63(6): 293-297 | DOI: 10.18832/kp201728

The Occurrence of Mycotoxins in Beers from Retail ShopsPeer-reviewed article

Sylvie BĚLÁKOVÁ, Simona WAWROSZOVÁ, Karolína BENEŠOVÁ
1 Výzkumný ústav pivovarský a sladařský, a. s., Sladařský ústav Brno, Mostecká 7, 614 00 Brno, Česká republika Research Institute of Brewing and Malting, Malting Institute Brno, Mostecká 7, 614 00 Brno, Czech Republic
2 Ústav chemie potravin a biotechnologií, Fakulta chemická, Vysoké učení technické v Brně, Purkyňova 118, 61200 Brno, Česká republika Institute of Food Science and Biotechnology, Faculty of Chemistry, Brno University of Technology, Purkyňova 118, 612 00 Brno, Czech Republic

In 2016, contents of mycotoxin deoxynivalenol, its metabolite deoxynivalenol-3-β-D-glucopyranoside and ochratoxin A, in beers from Czech, Polish and Slovak retail shops were studied. Totally, 30 beer samples were analyzed. Deoxynivalenol and its metabolite were determined using high performance liquid chromatography with mass detection (HPLC/MS) HPLC, and ultra-high performance liquid chromatography with fluorescence detection (UPLC/FLR) was employed for the determination of ochratoxin A. Deoxynivalenol was detected in 25 samples in the concentration range of 1.54 - 12.57 µg.l-1 and deoxynivalenol-3-β-D-glucopyranoside was present in 27 samples in the contamination range of 1.18 - 12.47 µg.l-1. Ochratoxin A was detected in the contamination range of 1.2 - 82.5 ng.l-1.

Keywords: beer, mycotoxins, deoxynivalenol, deoxynivalenol-3-β-D-glucopyranoside, ochratoxin A

Received: July 6, 2017; Accepted: October 17, 2017; Published: December 15, 2017 

References

  1. Amézqueta, S., González-Penas, E., Murillo-Arbizu, M., López de Cerain, A., 2009: Ochratoxin A decontamination: A review. Food Control, 20: 326-333. Go to original source...
  2. Anli, E., Alkis, M., 2010: Ochratoxin A and Brewing Technology: A Review. J.Inst.Brew., 116(1): 23-32. Go to original source...
  3. Basařová, G., Šavel, J., Basař, P., Lejsek, T., 2010: Pivovarství: Teorie a praxe výroby piva. VŠCHT v Praze. ISBN 978-80-7080-734-7.
  4. Baxter, E. D., Slaiding, I. R. and Kelly, B., 2001: Behavior of ochratoxin A in brewing. American Society of Brewing Chemists., 59: 98-100. Go to original source...
  5. Beer Statistics - 2016 Edition. In: The Brewers of Europe [online]. Belgium, 2016. [cit. 29. 3. 2017]. ISBN 978-2-9601382-7-6. Dostupné na www: http://www.brewersofeurope.org/site/media‑centre/index.php?doc_id=840&class_id=31&detail=true.
  6. Běláková, S., Benešová, K., Mikulíková, R., Svoboda, Z., 2011: Determination of ochratoxin A in brewing materials and beer by ultra performance liquid chromatography with fluorescence detection. Food Chemistry, 126 (1): 321-325. Go to original source...
  7. Běláková, S., Benešová, K., Mikulíková, R., Svoboda, Z., Čáslavský, J., 2013: Monitoring výskytu deoxynivalenolu v pivech z obchodní sítě v letech 2009-2012. Kvasny prum., 59 (10-11): 292-295. Go to original source...
  8. Běláková, S., Benešová, K., Mikulíková, R. a Svoboda, Z., 2015: Výskyt ochratoxinu A v pivech. Kvasny prum., 61(2): 34-37. Go to original source...
  9. Bennett, J. W., 1987: Mycotoxins, mycotoxicoses, mycotoxicology and mycopatologia. Mycopatologia, 100(1): 3-5. Go to original source...
  10. Berthiller, Fr., Sulyok, M., Krska, R., Schuhmacher, R., 2007: Chromatographic methods for the simultaneous determination of mycotoxins and their conjugates in ceereals. International Journal of Food Microbiology, 119(1-2): 33-37. Go to original source...
  11. Berthiller, F., Crews, C., Dall'Asta, C., De Saega, S., Haesaert, G., Karlovský, P., Oswald, I.P., Seefelder, W., Speijers, G., Stroka, J., 2013: Masked mycotoxins: A review. Molecular Nutrition & Food Research, 57: 165-186. Go to original source...
  12. Capriotti, A. L., Foglia, P., Gubbiotti, R., Roccia, C., Samperi, R., Laganà, A., 2010: Development and validation of a liquid chromatography/atmospheric pressure photoionization-tandem mass spectrometric method for the analysis of mycotoxins subjected to commission regulation (EC) No.1881/2006 In cereals. Journal of Chromatography A., 1217: 6044-6051. Go to original source...
  13. Gumus, T., Arici, M., Demirci, M., 2004: A Survey of Barley, Malt and Beer Contamination with Ochratoxin A in Turkey. Journal of the institute of brewing., 110(2): 146-149. Go to original source...
  14. Hajšlová, J., 2009: Mykotoxiny. Vědecký výbor fytosanitární a životního prostředí [online]. [cit. 9. 6. 2016]. Dostupné na www: http://www.phytosanitary.org/projekty/2009/Projekt1.pdf
  15. Inoue, T., Nagatomi, Y., Uyama, A., Mochizuki, N., 2013: Fate of mycotoxins during beer brewing and fermentation. Biosci. Biotechnol. Bichem., 77 (7): 1410-1415. Go to original source...
  16. Kostelanska, M., Hajslova, J., Zachariasova, M., Malachova, A., Kalachova, K., Poustka, J., Fiala, J., Scott, P. M., Berthiller, F.,Krska, R., 2009: Occurrence of Deoxynivalenol and its Major Conjugate, Deoxynivalenol-3-Glucoside, in Beer and Some Brewing Intermediates. Journal of Agricultural and Food Chemistry, 57: 3187-3194. Go to original source...
  17. Kostelanska, M., Zachariasova, M., Lacina, O., Fenclova, M., Kollos, A., Hajslova, J., 2011: The study of deoxynivalenol and its masked metabolites fate during the brewing process realised by UPLC-TOFMS method. Food Chemistry, 126: 1870-1876. Go to original source...
  18. Malíř, F., Ostrý, V., 2003: Vláknité mikromycety (plísně), mykotoxiny a zdraví člověka, 1.vydání, Mikada, Brno. ISBN: 80-7013-395-3.
  19. Malíř, F., Ostrý, V., Prohl-Leszkowicz, A., Novotná, E., 2014: Mycotoxin ochratoxin A and developmental and reproductive toxicity - an overview . Developmental and Reproductive Toxicology: 1-10.
  20. Mateo, R., Medina, A., Mateo, E. M., Mateo, F., Jiménez, M., 2007: An overview of ochratoxin A in beer and wine. International Journal of Food Microbiology, 119 (1-2): 79-83. Go to original source...
  21. Medina, A., Jiménez, M., Gimeno-Adelantado, J. V., Valle-Algarra, F. M., Mateo, R., 2005: Determination of ochratoxin A in beer marketed in Spain by liquid chromatography with fluorescence detection using lead hydroxyacetate as a clean-up agent. Journal of Chromatography A., 1083 (1-2): 7-13. Go to original source...
  22. Papadopoulou-Bouraoui, A., Vrabcheva, T., Valzacchi, S., Stroka, J., Anklam, E., 2004: Screening survey of deoxynivalenol in beer from the European market by an enzyme-linked immunosorbent assay. Food Additives and Contaminants, 21(6): 607-617. Go to original source...
  23. Ruprich, J., Ostry, V., 1995: Determination of the mycotoxin deoxynivalenol in beer by commercial Elisa tests and estimation of the exposure dose from beer for the population in the Czech Republic. Central European Journal of Public Health, 3(4): 224-229.
  24. Scott, P. M., Kanhere, S. R., 1995: Determination of ochratoxin A in beer. Food Addit. Contam., 12(4): 591-598. Go to original source...
  25. Scott, P. M., 1996: Mycotoxins transmitted into beer from contaminated grains during brewing. Journal of the AOAC International, 79: 875-882. Go to original source...
  26. Schothorst, R. C., Jekel, A. A., 2003: Determination of trichothecenes in beer by capillary gas chromatography with flame ionisation detection. Food Chemistry, 82(3): 475-479. ISSN 03088146. Go to original source...
  27. Varga, E., Malachova, A., Schwartz, H., Krska, R., Berthiller, F., 2013: Survey of deoxynivalenol and its conjugates deoxynivaleno-3-glucoside and 3-acetyl-deoxynivalenol in 374 beer samples. Food Additives and Contaminants., 30(1): 137-146. Go to original source...
  28. Velíšek, J., Hajšlová, J., 2009: Chemie potravin II. Havlíčkův Brod : nakladatelství OSSIS. ISBN: 978-80-86659-16-9.
  29. Wolf-Hall, C. E., 2007: Mold and mycotoxin problem endountered during malting and brewing. International Journal of Food Microbiology,119: 89-94. Go to original source...
  30. Yordanova, P., Vrabcheva, T., 2006: Ochratoxin A in bulgarian beer and wine. Advances in Bulgarian Science, 4: 12-19. ISSN 1312-6164.