Kvasny Prum. 2018; 64(1): 2-5 | DOI: 10.18832/kp201801

Quantification of β-glucans in Barley - ReviewPeer-reviewed article

Ondřej JIRSA, Irena SEDLÁČKOVÁ, Kateřina VACULOVÁ
Agrotest fyto, s.r.o., Havlíčkova 2787/121, Kroměříž, 767 01

The increasing interest in defining the content of β-glucans is related to the development of methods for their determination. Several methods of determination have been published. At present, the most widespread McCleary's enzymatic method is based on the specific hydrolysis of β-glucan by enzymes with β-glucanase activity and the determination of free glucose, which has become the official AACC, AOAC, and ICC method. The second most widely used method is the Flow Injection Analysis (FIA) method based on fluorescence detection of the Calcofluor complex with β-glucans. This method has become the official EBC method. In addition, modifications of the enzymatic method and other methods of determination - chromatographic and spectrometric - are surveyed.

Keywords: ječmen, β-glukany, enzymatická metoda, Calcofluor, HPLC

Received: June 28, 2017; Accepted: December 2, 2017; Published: February 15, 2018 

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