Kvasny Prumysl, 2012 (vol. 58), issue 4
New trends in liquid chromatography and their utilization in analysis of beer and brewery raw materials. Part 2. Determination of cis/trans- isomers -iso-α-acids in beer using Ultra Performance Liquid Chromatography.Peer-reviewed article
Jana OLŠOVSKÁ, Marie JURKOVÁ, Pavel ČEJKA
Kvasny Prum. 2012; 58(4): 94-99 | DOI: 10.18832/kp2012010 
The Ultra High Performance Liquid Chromatography (UHPLC), which uses the principle of separation on sub-2 μm particles, was tested for the separation and determination of steric forms of iso-α-acids in beer. The new UHPLC method was compared with the HPLC method, which is used for iso-α-acid determinations in RIBM. The comparison was performed with respect to the speed of analysis, separation efficiency, mobile phase consumption, and finally repeatability. The results of this study determined all the expected advantages of the UHPLC; while maintaining the resolution this method reduced analysis time 2.5 times compared to the previous...
Variability in phytic acid content in barley grain.Peer-reviewed article
Kateřina VACULOVÁ, Marta BALOUNOVÁ, František KVASNIČKA, Irena SEDLÁČKOVÁ, Jaroslava EHRENBERGEROVÁ, Eliška VÁCLAVÍKOVÁ, Milan POUCH
Kvasny Prum. 2012; 58(4): 100-108 | DOI: 10.18832/kp2012011
Around 65 % of phosphorus (P) in barley grain is in the form of phytic acid (PA) and phytates (PA salts), both forms negatively affect the usability of mineral substances and other important nutrients, thus participating in mineral deficiencies in human as well as livestock nutrition, and contributing to the pollution of the environment. Detection and creation of new varieties with a changed rate of both P forms in favor of digestible phosphate (Pi) is one of possible solutions. The submitted study dealt with the evaluation of variability in contents of PA and Pi in grains of selected spring and winter barley varieties, genetic resources and new spring...
Mycotoxicological quality of barley harvested in the Czech Republic in 2005-2010.Peer-reviewed article
Ivana POLIŠENSKÁ, Ondřej JIRSA, Pavel MATUŠINSKY
Kvasny Prum. 2012; 58(4): 109-114 | DOI: 10.18832/kp2012012
A representative set of barley samples collected in the Czech Republic in 2005-2010 was used to evaluate the occurrence of Fusarium mycotoxins deoxynivalenol (DON) and zearalenone (ZEA), the effect of agronomic factors on the level of mycotoxin contamination and the presence of four Fusarium species (F. avenaceum, F. culmorum, F. graminearum and F. poae). A significant correlation between the year and DON as well as ZEA content in barley, and significant effects of the preceding crop and variety on DON content were confirmed. The highest level of DON content was found in grain harvested in 2009, which was in correspondence with extremely frequent detection...