Kvasny Prum. 2007; 53(9): 273-276 | DOI: 10.18832/kp2007015

Determination of Barley Prolamins in Beer and Brewing Materials.Peer-reviewed article

Petr HULÍN, Pavel DOSTÁLEK, Igor HOCHEL
1 Ústav kvasné chemie a bioinženýrství, VŠCHT Praha, Technická 5, 166 28 Praha 6
2 Ústav Biochemie a Mikrobiologie, VŠCHT Praha, Technická 5, 166 28 Praha 6

The work deals with the possibilities of immunochemical detection of hordein fractions of barley proteins and possible cleavage derivates, or more precisely by characterization of monoclonal antibodies prepared for the purpose of a rapid, specific and sensitive determination of hordein proteins in the food industry, specifically in beer. A method of indirect competitive ELISA for barley prolamins has been developed and working concentrations of all immunoagents have been determined. Further to this, cross interactions of particular monoclonal antibodies with the proteins of the other cereals have been tested. The detection limit of the method was 2 ppm with the linear range of the calibration curve ranging from 1 to 100 ppm.The work deals with the possibilities of immunochemical detection of hordein fractions of barley proteins and possible cleavage derivates, or more precisely by characterization of monoclonal antibodies prepared for the purpose of a rapid, specific and sensitive determination of hordein proteins in the food industry, specifically in beer. A method of indirect competitive ELISA for barley prolamins has been developed and working concentrations of all immunoagents have been determined. Further to this, cross interactions of particular monoclonal antibodies with the proteins of the other cereals have been tested. The detection limit of the method was 2 ppm with the linear range of the calibration curve ranging from 1 to 100 ppm.

Keywords: prolamins, barley, beer

Published: September 1, 2007 

References

  1. Bermudo-Redondo, M.C., Griffin, P.B., Ellis, H.J., Ciclitira, P.J., O'Sullivan, C.K.: Monoclonal antibody-based competitive assay for the sensitive detection of coeliac disease toxic prolamins. Anal. Chim. Acta 551, 2005, 105-114. Go to original source...
  2. Chirdo, F.G., Anón, M.C., Fossati, C.A.: Optimization of a competitive ELISA with polyclonal antibodies for quantification of prolamins in foods. Food Agricult. Immunol. 7, 1995, 333-343. Go to original source...
  3. Brett, G.M., Mills, E.N.C., Goodfellow, B.J.: Epitope Mapping Studies of Broad Specificity Monoclonal Antibodies to Cereal Prolamins.: J. Cereal Sci. 29, 1999, 117-128. Go to original source...
  4. Dostálek, P., Hochel, I., Méndez, E., Hernando, A., Gabrovská, D.: Immunochemical determination of gluten in malts and beers. Food Addit. Contam. 23, 2006, 1074-1078. Go to original source...
  5. Kanerva, P., Sontag-Strohm, T., Lehtonen, P.: Determination of prolamins in beers by ELISA and SDS-PAGE. J. Inst. Brew. 111, 2005, 61-64. Go to original source...
  6. Laemli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 1970, 680-685. Go to original source...

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