Kvasny Prum. 2011; 57(1): 8-12 | DOI: 10.18832/kp2011002

Monitoring of Methionine in Czech and Foreign Beers.Peer-reviewed article

Renata MIKULÍKOVÁ, Zdeněk SVOBODA, Karolína BENEŠOVÁ, Sylvie BĚLÁKOVÁ, Ivana MÁROVÁ
1 Výzkumný ústav pivovarský a sladařský, a. s., Sladařský ústav, Mostecká 7, 614 00 Brno
2 FCH VUT v Brně, Purkyňova 118, 612 00 Brno

Volatile sulphur substances are precursors of sulphur amino acids, first of all sulphur amino acid metionine. Content of methionine was studied in Czech and foreign beers obtained from the retail shops. The technique for the determination of methionine in beer was optimized using the gas chromatography method with a selective flame photometric detector. Following validation parameters were achieved: LOD 0.80 mg.l-1, LOQ 2.5 mg.l-1, R2 0.99974, RSD 13 %.Volatile sulphur substances are precursors of sulphur amino acids, first of all sulphur amino acid metionine. Content of methionine was studied in Czech and foreign beers obtained from the retail shops. The technique for the determination of methionine in beer was optimized using the gas chromatography method with a selective flame photometric detector. Following validation parameters were achieved: LOD 0.80 mg.l-1, LOQ 2.5 mg.l-1, R2 0.99974, RSD 13 %.

Keywords: methionine, GC/FPD, derivatization, beer

Received: November 10, 2009; Accepted: January 7, 2010; Published: January 1, 2011 

References

  1. Vodrážka, Z.: Biochemie. 1. vyd. Praha: Academia, 2002, 508 s. ISBN 80-200-0600-1.
  2. Voet, D., Voetová, J.: Biochemie. 1. vyd., Praha: Victoria Publishing, a. s., 1995, 1325 s., ISBN 80-85605-44-9.
  3. Basařová, G., Šavel, J., Basař, P., Lejsek, T.: Pivovarství teorie a praxe výroby piva. 1. vyd. Praha: VŠCHT, 2010, 863 s. ISBN 97880-7080-734-7.
  4. Vacek, J., Onofrejová, L., Klejdus B., Kubáň V.:Využití kapalinové chromatografie založené na hydrofilních interakcích pro separace polárních látek. Chem. Listy 103, 2009, 381-385.
  5. Langrock, T., Czihal, P., Hoffmann, R.: Amino acid analysis by hydrophilic interaction chromatography coupled on-line to electrospray ionization mass spectrometry. Amino Acids, 30, 2006, 291-297. Go to original source...
  6. Hušek, P.: Chloroformates in gas chromatography as general purpose derivatizing agents, J. Chromatogr. B, 717, 1998, 57-91. Go to original source...
  7. Hušek, P., Matucha, P., Vránová, A., Šimek, P.: Simple plasma work-up for fast chromatographic analysis of homocysteine, cysteine, methionine and aromatic amino acids, J. Chromatogr. B, 789, 2003, 311-322. Go to original source...
  8. Myung S., Kim M., Min H., Yoo E., Kim K.: Determination of homocysteine and its related compounds by SPME/ GC/ MSD, J. Chromatogr. B, 727, 1999, 1-8. Go to original source...
  9. Mayadunne, R., Nguyen, T.-T., Marriott, P., J.: Amino acid analysis by using comprehensive two-dimensional gas chromatography. Analytical and Bioanalytical Chemistry 382, 2005, 836-847. Go to original source...
  10. Erbe, T., Brückner, H.: Chromatographic determination of amino acid enantiomers in beers and raw materials used for their manufacture. J. Chromatogr. A, 881, 2000, 81-91. Go to original source...
  11. Mikulíková, R., Svoboda, Z., Benešová, K., Běláková, S.: Stanovení methioninu ve sladu. Kvasny Prum. 55, 2009, 310-314. Go to original source...
  12. Barek, J. a kol.: Metrologická terminologie v chemii, Chem. Listy 94, 2000, 439-444.
  13. Spektroskopická společnost Jana Marka Marci: Nejistota a nezávaznost výsledků spektroskopických metod, 2001, ISBN 80-7080-447-5.

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