Kvasny Prum. 2011; 57(10): 366-370 | DOI: 10.18832/kp2011039

Hops - an abundant source of antioxidants. Methods to assessment of antioxidant activity of hop matrix.Peer-reviewed article

Marie JURKOVÁ, Vladimír KELLNER, Danuša HAŠKOVÁ, Jiří ČULÍK, Pavel ČEJKA, Tomáš HORÁK, Josef DVOŘÁK
Výzkumný ústav pivovarský a sladařský, a. s., Pivovarský ústav Praha, Lípová 15, 120 44 Praha 2

The contribution of individual hop compounds to the total antioxidant activity was studied by HPLC method with CoulArray detection in broad scale of the potentials 250-900 mV. The compounds with the same electrochemical manners as epicatechin and catechin and oligomeric proanthocyanogens (OPCs) related to catechin were distinguished from the other compounds according to their electrochemical responses on lower potential (500 mV). The total content of these OPCs including catechin and epicatechin was determined as an equivalent of concentration of the catechin used as an external standard. The results were correlated partly with the results obtained by using of the group analysis for determination of the total polyphenol and for the determination of the anthocyanogens, partly with results obtained by using of the modern method electron spin resonance (ESR) for determination of the antioxidant activity.The contribution of individual hop compounds to the total antioxidant activity was studied by HPLC method with CoulArray detection in broad scale of the potentials 250-900 mV. The compounds with the same electrochemical manners as epicatechin and catechin and oligomeric proanthocyanogens (OPCs) related to catechin were distinguished from the other compounds according to their electrochemical responses on lower potential (500 mV). The total content of these OPCs including catechin and epicatechin was determined as an equivalent of concentration of the catechin used as an external standard. The results were correlated partly with the results obtained by using of the group analysis for determination of the total polyphenol and for the determination of the anthocyanogens, partly with results obtained by using of the modern method electron spin resonance (ESR) for determination of the antioxidant activity.

Keywords: oligomeric proanthocyanogens (OPCs), HPLC-CoulArray, ESR - DPPH

Received: June 12, 2011; Accepted: August 29, 2011; Published: October 1, 2011 

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