Kvasny Prum. 2010; 56(3): 127-130 | DOI: 10.18832/kp2010017

Antioxidant activity of superoxide dismutase in spring barley grain.Peer-reviewed article

Natálie BŘEZINOVÁ BELCREDI, Jaroslava EHRENBERGEROVÁ, Kateřina VACULOVÁ
1 Mendelova univerzita v Brně, Zemědělská 1, 613 00 Brno
2 Zemědělský výzkumný ústav Kroměříž, s. r. o., Havlíčkova 2787/121, 767 01 Kroměříž

Activity of antioxidant enzyme superoxide dismutase (SOD) was assessed in grain samples of 12 varieties and lines of spring barley grown in two localities (Žabčice, Kroměříž). In 2005, 2007, and 2008 field experiments were established in the locality Žabčice in two growing systems (with the application of pesticides, without application of pesticides) and in the locality Kroměříž without the application of pesticide. Activity of SOD was significantly affected by the varieties/lines, localities, years and their interactions. The treatment method did not affect the SOD activity statistically significantly. Significantly higher activity SOD (P = 0.05) was in the hull-less line KM 1057 (143 U.g-1 in the dry matter) compared to the total set from both the growing localities. On the contrary, significantly lower activity was recorded in the hull-less variety Merlin (62 U.g-1 in the dry matter) compared to the other varieties/lines. Malting varieties recommended for production of Czech Beer, Bojos and Tolar, also had higher SOD activity in grain.Activity of antioxidant enzyme superoxide dismutase (SOD) was assessed in grain samples of 12 varieties and lines of spring barley grown in two localities (Žabčice, Kroměříž). In 2005, 2007, and 2008 field experiments were established in the locality Žabčice in two growing systems (with the application of pesticides, without application of pesticides) and in the locality Kroměříž without the application of pesticide. Activity of SOD was significantly affected by the varieties/lines, localities, years and their interactions. The treatment method did not affect the SOD activity statistically significantly. Significantly higher activity SOD (P = 0.05) was in the hull-less line KM 1057 (143 U.g-1 in the dry matter) compared to the total set from both the growing localities. On the contrary, significantly lower activity was recorded in the hull-less variety Merlin (62 U.g-1 in the dry matter) compared to the other varieties/lines. Malting varieties recommended for production of Czech Beer, Bojos and Tolar, also had higher SOD activity in grain.

Keywords: barley, enzyme, superoxide dismutase, SOD, localities, treatment

Received: November 19, 2009; Accepted: January 7, 2010; Published: March 1, 2010 

References

  1. Bhatty, R. S.:The Potential of Hull-less Barley - A Review. Cereal Chem. 63, 1986, 97-103.
  2. Boivin, P.: Pro- and anti-oxidant enzymatic activity in malt. Cerevisia 20, 2001, 109-115.
  3. Piterková, J., Tománková, K., Luhová, L., Petřivalský, M., Peč, P.: Oxidativní stres: Lokalizace tvorby aktivních forem kyslíku a jejich degradace v rostlinném organismu. Chem. Listy 99, 2005, 455-466.
  4. Meng de J., Lu, J., Fan, W., Dong, J. J., Lin, Y., Shan, L. J.: Control of Superoxide Dismutase Activity During Malting Using Plackett-Burman and Box-Behnken Experimental Design and Its Effect on Reducing Power ofWort.J.Inst.Brew.113, 2007, 365-373. Go to original source...
  5. Havlová, P.: Hydrolytické a oxidoredukční enzymy ječného sladu. ÚZPI, Praha, 1999.
  6. Racek, J.: Oxidační stres a možnosti jeho ovlivnění. Houdek L., Lipovská D. (eds.), 1.vyd., Praha: Galén, 2003, 90 s.
  7. Bamforth, C.W., Parsons, R.: New Procedures to Improve the Flavor Stability of Beer. J. Am. Soc. Brew. Chem. 43, 1985, 197-202. Go to original source...
  8. Bowler, C., Montagu, V. M., Inze, D.: Superoxide Dismutase and Stress Tolerance. Annual Review of Plant Physiol. and Plant Mol. Biol. 43, 1992, 83-116. Go to original source...
  9. Zima, T., Štípek, S., Tesař, V., Pláteník, J., Crkovská, J.: Volné radikály - reaktivní formy kyslíku, antioxidační látky a antioxidační terapie. Remedia 6, 1996, 35-58.
  10. Giannopolitis C. N., Ries S. K.: Superoxide Dismutase I. Occurrence in Higher Plants. Plant Physiol. 59, 1977, 309-314. Go to original source...
  11. Bamforth, C. W.: Superoxide-dismutase in Barley. J. Inst. Brew. 89, 1983, 420-423. Go to original source...
  12. Belcrediová, N., Ehrenbergerová, J., Havlová P.: Enzym superoxid dismutasa v zrnu ječmene a sladu. Acta Univ. Agric. et Silvic. Mendel. Brun. 54, 2006, 7-14. Go to original source...
  13. Březinová Belcredi, N., Ehrenbergerová, J., Prýma, J., Havlová, P.: Stanovení aktivity enzymu superoxiddismutasy pomocí Soupravy Ransod v rostlinném materiálu. Chem. Listy 101, 2007, 504-508.
  14. Březinová Belcredi, N., Kopáček, J., Ehrenbergerová, J., Prýma, J., Paulíčková, I., Vaculová, K.: Využití zelené biomasy ječmene jarního.In Cerkal, R., Vejražka, K., Hrstková, P., Středa, T.- MZLU pěstitelům 2007. Žabčice. Brno: MZLU Brno, 2007, 28-31.
  15. Malachová, A., Hajšlová, J., Ehrenbergerová, J., Kostelanska, M., Zachariášová, M., Urbanová, J., Cerkal, R., Šafránková, I., Marková, J., Březinová Belcredi, N., Vaculová, K., Hrstková, P.: Fusariové mykotoxiny v zrnu ječmene jarního a jejich přenos do sladu. Kvasny Prum. 56, 2010, 131-137. Go to original source...
  16. Březinová Belcredi, N., Marková, J., Ehrenbergerová, J., Prýma J., Vaculová, K., Lancová, K., Hajšlová, J.:Antioxidant and Mycotoxins in Barley Grain. In Ugarćić-Hardi, Ž. Proceedings of 4th International Congress FLOUR - BREAD'07 and 6th Croatian Congress of Cereal Technologists. J. J. Strossmayer University of Osijek, Croatia: Faculty of Food Technology, 2008, 248-253.
  17. Ehrenbergerová, J., Březinová Belcredi, N., Kopáček, J., Melišová, L., Hrstková, P., Macuchová, S., Vaculová, K., Paulíčková, I.: Antioxidant enzymes in barley green biomass. Plant Food Hum. Nutr. 64, 2009, 122-128. Go to original source...
  18. Havlová, P., Belcrediová, N., Ehrenbergerová, J.: Monitoring of Superoxide dismutase Enzyme Activity in Barley Grain and Malt. In Horna, A. The Abstracts Book Vitamins 2005 - Targeted Nutritional Therapy., 1. vyd. Pardubice, Czech Republic: Univerzita Pardubice, 2005, s. 83.
  19. Gupta, A. S., Heinen, J. L., Holaday, A. S., Burke, J. J., Allen, R. D.: Increased Resistance to Oxidative Stress in Transgenic Plants that Overexpress Chloroplastic Cu/Zn Superoxide Dismutase. In Proceedings of the National Academy of Sciences of the United States of America 90, 1993, 1629-1633. Go to original source...
  20. Březinová Belcredi, N., Ehrenbergerová, J., Benešová, K., Vaculová, K: Variabilita aktivity vitamínu E v zrnu ječmene jarního. Kvasny Prum. 56, 2010, 88-92. Go to original source...