Kvasny Prumysl, 2010 (vol. 56), issue 3
Possibility of evaluation of drought tolerance in barley.Peer-reviewed article
Ludmila HOLKOVÁ, Lucie MELIŠOVÁ, Marta BRADÁČOVÁ, Pavlína MIKULKOVÁ, Jaroslava EHRENBERGEROVÁ
Kvasny Prum. 2010; 56(3): 118-122 | DOI: 10.18832/kp2010015 
Breeding of drought-tolerant varieties, which is steadily gaining in importance for improving crop yields and health condition of economically important crops, is based on evaluation and efficient selection of tolerant genotypes. The assessment of plant sensitivity to drought is not simple owing to the complex character of this trait. We compared the stress responses of five different barley genotypes with different sensitivity (Tadmor, Malz, Amulet, Bojos and Jersey) exposed to drought stress by using a molecular biological method based on evaluating the activity of two dehydrin genes, Dhn1 and Dhn4. The efficiency of the method was verified in laboratory...
Impact of gene expression of chosen genes involved in the biosynthetic pathway of vitamin E on the total vitamin E content and composition in grain of spring barley.Peer-reviewed article
Michal KOSAŘ, Ludmila HOLKOVÁ, Natálie BŘEZINOVÁ BELCREDI, Jaroslava EHRENBERGEROVÁ
Kvasny Prum. 2010; 56(3): 123-126 | DOI: 10.18832/kp2010016
Vitamin E has a very important role in human nutrition. Sources of vitamin E are i.e. oils, nuts and also cereals. The highest vitamin E content among cereals was detected in barley. There are two enzymes localized in the strategic location of biosynthetic pathway of vitamin E : 4-hydroxyphenylpyruvate dioxygenase enzyme (HPPD) and homogentisategeranylgeranyl transferase (HGGT). HPPD could be considered as one of the essential factors which could control activity of the whole biosynthetic pathway due to its localization in the beginning of biosynthetic pathway. HGGT is the only enzyme which differs in the biosynthetic pathway of tocopherols and tocotreinols...
Antioxidant activity of superoxide dismutase in spring barley grain.Peer-reviewed article
Natálie BŘEZINOVÁ BELCREDI, Jaroslava EHRENBERGEROVÁ, Kateřina VACULOVÁ
Kvasny Prum. 2010; 56(3): 127-130 | DOI: 10.18832/kp2010017
Activity of antioxidant enzyme superoxide dismutase (SOD) was assessed in grain samples of 12 varieties and lines of spring barley grown in two localities (Žabčice, Kroměříž). In 2005, 2007, and 2008 field experiments were established in the locality Žabčice in two growing systems (with the application of pesticides, without application of pesticides) and in the locality Kroměříž without the application of pesticide. Activity of SOD was significantly affected by the varieties/lines, localities, years and their interactions. The treatment method did not affect the SOD activity statistically significantly. Significantly higher activity SOD (P = 0.05)...
Fusarium mycotoxins in spring barley and their transfer into malt.Peer-reviewed article
Alexandra MALACHOVÁ, Jana HAJŠLOVÁ, Jaroslava EHRENBERGEROVÁ, Marta KOSTELANSKÁ, Milena ZACHARIÁŠOVÁ, Jana URBANOVÁ, Radim CERKAL, Ivana ŠAFRÁNKOVÁ, Jaroslava MARKOVÁ, Kateřina VACULOVÁ, Pavlína HRSTKOVÁ
Kvasny Prum. 2010; 56(3): 131-137 | DOI: 10.18832/kp2010018
Fusarium mycotoxins are secondary metabolites of microscopic filamentous fungi of Fusarium genus. This paper summarises current knowledge on the field of mycotoxin contamination of barley, mycotoxin transfer into malt and compares them with results achieved within 2005-2008 years on the set of 12 spring barley cultivars grown in low-input and conventional agricultural systems in Žabčice a Kroměříž.Fusarium mycotoxins are secondary metabolites of microscopic filamentous fungi of Fusarium genus. This paper summarises current knowledge on the field of mycotoxin contamination of barley, mycotoxin transfer into malt and compares them with results achieved...
Seed mycoflora of malting varieties and lines of spring barley in localities Kroměříž and Žabčice.Peer-reviewed article
Ivana ŠAFRÁNKOVÁ, Jaroslava MARKOVÁ, Martin KMOCH
Kvasny Prum. 2010; 56(3): 138-144 | DOI: 10.18832/kp2010019
Determination of protective measures for grain destined for storage requires the knowledge of the way and time of colonization with individual microbial pathogens, most of which are fungi that strongly affect the quality of production. The most frequent species colonizing barley seeds are fungi of genera Alternaria, Cladosporium, Epicoccum, Fusarium, Aspergillus and Penicillium. The current fungicidal protection does not guarantee sufficient protection against fusarioses of seed and ears. The most efficient fungicides against ear fusarioses are formulas containing sterol demethylation inhibiting (DMI) fugicides; when using them it is important to know...
The influence of barley origin and malting technology on fe - rulic acid content in barley and malt.Peer-reviewed article
Alexandr MIKYŠKA, Josef PROKEŠ, Sylvie BĚLÁKOVÁ, Josef ŠKACH, Danuša HAŠKOVÁ
Kvasny Prum. 2010; 56(3): 145-151 | DOI: 10.18832/kp2010020
Ferulic acid is an important phenolic acid contained in barley. It is an antioxidant with a potential impact on sensorial stability of beer. On the other hand, 4-vinyl guaiacol can be formed from free ferulic acid either by thermal decarboxylation in the course of kilning, mashing and wort boiling or by enzymic decarboxylation during beer fermentation. In six years trials was approved dependence of ferulic acid in barley on its genetical properties and important dependence on climatic circumstances during growth as well as during year crop. Free ferulic acid monitoring in laboratory wort approved dependence of its content on barley variety, year crop...
Zinc - effect on the spring barleyʼs plant and roots growth, grain technological quality, and yeast fermentation.Peer-reviewed article
Radim CERKAL, Luděk HŘIVNA, Pavel RYANT, Josef PROKEŠ, Natálie BŘEZINOVÁ BELCREDI, Karel VEJRAŽKA, Michaela MICHNOVÁ, Gregor TOMÁŠ
Kvasny Prum. 2010; 56(3): 152-159 | DOI: 10.18832/kp2010021
In 2005-2008, the effect of zinc foliar application (0.5 kg.ha-1 in DC 31 and 55 growth stage in the form of the Zinran fertilizer containing sulphate and zinc oxide - 19.8 and 52.3 %) on the size of the plants root system (its electric capacity in DC 23 and 57 was measured), the yield and quality of grain, and the course of the sweet wort fermentation process of six spring barley varieties (Bojos, Jersey, Kompakt, Malz, Sebastian, Tolar) was studied. Varietal differences were found in regard to the impact of zinc on the root system development. The grain yield rose by 3% on average after zinc application and so did grain over sieve 2.5 mm (by 9%)...
Barley and malt polyphenols and their antioxidant properties.Peer-reviewed article
Markéta DVOŘÁKOVÁ, Pavel DOSTÁLEK, Zuzana SKULILOVÁ, Marie JURKOVÁ, Vladimír KELLNER, Luis F. GUIDO
Kvasny Prum. 2010; 56(3): 160-163 | DOI: 10.18832/kp2010022
Ten different barley cultivars and their corresponding malts were analysed for monomeric and oligomeric flavan-3-ols content by using high-performance liquid chromatography ultraviolet detection-electrospray ion trap mass spectrometry. The Folin-Ciocalteau and the vanillin spectrophotometric assays were used for the assessment of the total polyphenol and total flavan-3-ol content respectively, and the antioxidant activity was determined by DPPH and FRP. Catechin and prodelphinidin B3 were respectively the major monomeric and dimeric flavan-3-ols. Moreover, prodelphinidin B3 was shown to be the main contributor for the radical scavenging activity both...
Simultaneous determination of iso-alpha acids in their cis- and transforms and tetrahydroiso-alpha acids.Peer-reviewed article
Marie JURKOVÁ, Tomáš HORÁK, Jiří ČULÍK, Pavel ČEJKA, Vladimír KELLNER
Kvasny Prum. 2010; 56(3): 163-166 | DOI: 10.18832/kp2010023
The elaborated method enables the simultaneous determination of stereoisomer forms of iso-alpha acids and tetrahydroiso-alpha acids using very modest way of separation on reverse phase on C18 column mobile phase without buffers. The groups of analysed compounds elute separately. The rests of untransformated alpha acids to iso-alpha acids do not obstruct the analysis.The elaborated method enables the simultaneous determination of stereoisomer forms of iso-alpha acids and tetrahydroiso-alpha acids using very modest way of separation on reverse phase on C18 column mobile phase without buffers. The groups of analysed compounds elute separately. The rests...
Study of an influence of beer colloidal stabilization by sorbent Polyclar 10 on polyphenolic antioxidants and sensorial stability of beer.Peer-reviewed article
Alexandr MIKYŠKA, Danuša HAŠKOVÁ, Jíří ČULÍK, Marie JURKOVÁ, Pavel ČEJKA
Kvasny Prum. 2010; 56(3): 167-174 | DOI: 10.18832/kp2010024
The influence of beer colloidal stability by polyphenols sorbent treatment on polyphenols composition, antioxidant activity of beer, carbonyls content and sensorial quality of fresh and three months stored beer was studied in trial brews (200 L) hopped either by aroma or bitter hops. A part of each brew was stabilized by the use of two different doses of sorbent Polyclar 10. Content of both complex polyphenols and content of some free phenol compounds, flavanoids and p-hydroxybenzoic acid derivates decreased by stabilizing action. It was found out ESR-DPPH antioxidant activity decrease only in 5-15 % after 15-40 % polyphenols of investigated groups...