Kvasny Prumysl, 1984 (vol. 30), issue 2
New methods for malt checking. Determination of α-amylase in malt.Peer-reviewed article
M. NENTWICHOVÁ, A. DOLEŽALOVÁ
Kvasny Prum. 1984; 30(2): 25-28 | DOI: 10.18832/kp1984005
A description of the EBC method for α-amylase determination is made. Also methods based on a principle of chromogenous substrates (Phadebas, Spofa-Test) were tested. The correlation among values of α-amylase and the other important parameters of malt quality was calculated. A good correlation was found with RE at 45°C(R = 0.522+) and proteins (R = 0.495+). The correlation among the values of α-amylase and the others analytical criterions is demonstrated in the plot.(In Czech, English summary only)
Intensification of malting using bioregulation.Peer-reviewed article
V. TODOROVÁ
Kvasny Prum. 1984; 30(2): 29-31 | DOI: 10.18832/kp1984006
The author observed changes of cytolytic modification during a germination of barley using the following bioregulators: indoyl-3-acetic acid (heteroauxin), IOK, the complex enzyme preparate containing α-amylase and β-glucanase and the complex enzyme preparate B with proteolytic activity without amylolytic and cytolytic activities. In the concentration used the IOK preparate accelerated cytolysis of a barley germination and stimulated protein splitting. No effect on the content of α-amylase was observed. A small increase of diastatic power. The complex enzyme preparate A added supported cytolysis and increased α-amylolytic activity...
Production of single cell protein from whey.Peer-reviewed article
J. ŠANDULA, L. MASLER, A. VOJTKOVÁ
Kvasny Prum. 1984; 30(2): 31-34 | DOI: 10.18832/kp1984007
The article deals with fermentation of acid and sweet whey, and ultrafiltered whey by yeast. Some of yeast species are able to utilize lactose and produce amount of biomass rich in protein. The yeasts were grown by batch and continuous cultivation, the optimum conditions for fermentation as temperature, pH, nutrient concentration and aeration were determined. The best results were achieved with strains Candida humicola, Torulopsis candida, Kluyveromyces lactis and Kluyveromyces fragilis, yielding 27-33 g dry biomass per liter of whey. The protein content of biomass was found to be 48-57% and the amount of protein was increased...
Maintaining of yeast at very low temperatures.Peer-reviewed article
A. KOCKOVÁ-KRATOCHVÍLOVÁ
Kvasny Prum. 1984; 30(2): 35-38 | DOI: 10.18832/kp1984008
The article describes experiences with a maintaining of yeast at very low temperatures using liquid nitrogen. This procedure permits to achieve the temperature of-196,8 °C. Comparing with other methods, this procedure is more expensive but its great advantage is in the relatively high viability of cells (62 -95 %).(In Czech, English summary only)
Effect of interstage mixing on growth and metabolic activity of yeasts in multistage culture systems.Peer-reviewed article
J. PÁCA
Kvasny Prum. 1984; 30(2): 38-42 | DOI: 10.18832/kp1984009
The significance of the interstage mixing on growth characteristics and metabolic activity of cells Candida utilis at different levels of ethanol in the cultivation broth was studied. The experiments were performed in a multistage tower fermentor and in fermentors in series. The effect of the interstage mixing can be evaluated from comparison of results obtained from both culture systems under conditions of geometrical similarity, identity of oxygen transfer rate and identity of dilution rate per stage. Cells were cultivated on a synthetic medium with constant values of dilution rate, temperature and pH of the medium. It was demonstrated that...