Kvasny Prum. 1985; 31(10): 232-236 | DOI: 10.18832/kp1985056

Maintenance of production strains of baker's yeasts.Peer-reviewed article

J. PÁSKOVÁ, J. FABIÁN, A. KOCKOVÁ-KRATOCHVÍLOVÁ

A conservation of the production strain of baker's yeast by lyophilisation and by ultralow temperature in liquid nitrogen was tested. After lyophilisation in a diluted serum with 5 % lactose 11,3 % of the cells survived. The thermal test performed by a heating of evacuated sealed ampoules to80 °Cfor 30 min showed the survival of 1,6 % of the cells. The lyophylised cultures showed no worse technological properties (on laboratory and plant scale) during fermentations. Only an increased variability of the biomass growth and the ability to ferment maltose was determined on a base of the statistical evaluation. The preservation by the ultralow temperature using liquid nitrogen survived 44 % of the cells in a medium with the calf serum, in a medium with mannane 37 % of the cells. The cultures preserved by liquid nitrogen can be used direct as the inoculum for plant fermentation. However, the cultures preserved by lyophilisation cannot be used directly. Here, a selection of the suitable variety has to be performed and thereafter the isolates could be tested on a rotary shaking machine.
(In Czech, English summary only)

Keywords: NOT AVAILABLE

Published: October 1, 1985